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KMID : 0903520070500030204
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2007 Volume.50 No. 3 p.204 ~ p.209
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Isolation and Identification of Inhibitory Compound from Crataegi Fructus on α-amylase and α-glucosidase
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Kim Jeung-Hoan
Cho Young-Je
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Abstract
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The objective of this research was to evaluate the inhibitory activities of phenolic compounds isolated from Crataegi Fructus on α-amylase and α-glucosidase. The content of total phenolic compounds of water extract from Crataegi Fructus was 22.5 mg/g. The inhibitory activity of the water extract (200 μg/ml) from Crataegi Fructus on α-amylase and α-glucosidase was determined to be 100% and 82.6%, respectively. Isolation of inhibitory compounds was carried out on Sephadex LH- 20 and MCI-gel CHP-20 column chromatography using a gradient elution procedure of increasing MeOH in H2O. The chemical structure of the inhibitory compound against α-amylase and α-glucosidase was confirmed as chlorogenic acid by spectroscopic analysis of FAB-MS, NMR and IR spectrum.
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KEYWORD
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α-Amylase, Crataegi Fructus, α-glucosidase, MCI-gel CHP-20, Sephadex LH-20
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